VARIASI VOLUME DAN WAKTU PEWARNAAN WRIGHT-GIEMSA TERHADAP KUALITAS MORFOLOGI SEL POLIMORFONUKLEAR

Zaky Wahyudin, Nur Aini Hidayah Khasanah

Sari


Abstract : Peripheral Blood Smear (PBS) examination is a crucial method for evaluating blood cell morphology, including polymorphonuclear (PMN) leukocytes, whose appearance is highly dependent on staining quality. Wright–Giemsa staining is widely used because it provides excellent contrast between the nucleus, cytoplasm, and cytoplasmic granules. This study aimed to determine the effect of staining volume and staining time on the morphological quality of PMN cells. A true experimental study with a post-test-only design was conducted using six EDTA-anticoagulated blood samples that met the inclusion criteria. Three staining volumes (1.5 mL, 2.0 mL, and 2.5 mL) and three staining times (8, 12, and 16 minutes) were evaluated. The Kruskal-Wallis test demonstrated that variations in staining volume and staining time significantly affected PMN morphological quality (p < 0.05). Post hoc Mann–Whitney analysis showed that the optimal staining combinations for basophils and eosinophils were 8 minutes–2.0 mL, 12 minutes–1.5 mL, 12 minutes–2.5 mL, 16 minutes–1.5 mL, and 16 minutes–2.0 mL, producing optimal contrast of nuclear, cytoplasmic, and granule staining. For neutrophils, the optimal combinations were 8 minutes–2.0 mL, 12 minutes–1.5 mL, and 16 minutes–1.5 mL. The 8-minute–2.0 mL combination is recommended as the optimal staining condition because it provides high-quality staining while minimizing staining time and reagent consumption. In contrast, the 16-minute–2.5 mL combination resulted in over-staining, making PMN morphology difficult to evaluate. Further studies should investigate staining times shorter than 8 minutes and a wider range of staining volumes to determine the minimum conditions required for optimal staining quality.

Keywords: peripheral blood smear, polymorphonuclear cell morphology, staining volume, staining time, Wright-Giemsa.


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Anggreani, L., Aprianie, W., Hidayati, L., & Romaidha, I. (2025). Morfologi Sel Darah pada Sadt Tipis Menggunakan Ekstrak Bunga Senduduk (Melastoma malabathricum L.). Jurnal Kesehatan Tambusai, 6, 4999–5007.

Bodzas, A., Kodytek, P., & Zidek, J. (2023). A High-Resolution Large-Scale Dataset of Pathological and Normal White Blood Cells. Scientific Data, 1–5.

Fathun, D., Anggraeni, R., & Suryanto. (2025). Gambaran Morfologi Sel Granulosit dengan Pewarnaan Wright, Giemsa, dan May Grunwald-Giemsa. Jurnal Kedokteran dan Kesehatan Mahasiswa Malikussaleh, 4(4), 106–115.

Horobin, R. W. (2010). How Romanowsky Stains Work and Why They Remain Valuable — Including a Proposed Universal Romanowsky Staining Mechanism and a Rational. Biotechnic & Histochemistry, 86(1), 36–51.

Indrawan, D. W., Nailufar, Y., Rahmawati, Y., & Yogyakarta, U. A. (2025). Literature Review : Efektivitas Variasi Konsentrasi dan Waktu Inkubasi Giemsa pada Kualitas Pewarnaan Apusan Darah Tepi. Jurnal Medika Indonesia, 6, 7–16.

Kaza, N., Ojaghi, A., & Robles, F. E. (2022). Virtual Staining, Segmentation, and Classification of Blood Smears for Label-Free Hematology Analysis. BME Frontires, 2022, 14.

Khasanah, N. A. H., Husen, F., & Yuniati, N. I. (2023). Pewarnaan Sediaan Apusan Darah Tepi (SADT) Menggunakan Infusa Bunga Telang (Clitorea ternatea). Jurnal Bina Cipta Husada, XIX(1), 67–76.

Kiernan, J. A. (2010). Ask the Expert on Chemical Reactions and Staining Mechanisms What is the Difference Argyrophilic Reactions. Departement of Anatomy and Cell Biology, 127–136.

Muflihah, A. I., Destiawan, R. A., Wijaya, A. F., Yulia, L., Sufi, Q. N., Camelia, L., Azizah, N., Makki, A. A., & Imroatul, A. (2024). Gambaran Morfologi Sel Neutrofil pada Pewarnaan Giemsa dengan Variasi Waktu pada Larutan Pengencer Akuades. Jurnal Ilmiah Analis Kesehatan, 10(1), 126–135.

Nur, I., Uslafiah Anwar, A., Hartini, S., Dwi, D., & Prihandono, S. (2023). Gambaran Pewarnaan Giemsa, Wright dan Wright-Giemsa pada Slide Apusan Darah Tepi. MMLTJ(Mahakam Medical Laboratory Technology Journal), 3(1), 50–59.

Nurjanah, M. H., Azizah, M. I. F., Amirah, Djami, S. W., Novilla, A., Manik, S. E., Sulasmi, Rakhmawati, A., Maulidiyanti, E. T. S., Dekayana, A., Widaystuti, R., Ayanti, B. P., Agamonanza, F., Noviar, G., Arsyad, M., Ersanto, N., Bariyah, K., Marsudi, T. L. O., & Aliviameita, A. (2025). Hematologi Teknologi Laboratorium Medis.

Oktiyani, N., Muhlisin, A., Roebiakto, E., & Norsiah, W. (2023). Utilization of Alternative Buffer Solutions for Staining Thin Blood smears by the Giemsa , Wright stain and Romanowsky method. Jurnal Tropical Health and Medical Research, 5(1), 34–45.

Potutu, N. W. R. (2022). Optimalisasi Waktu Pewarnaan Giemsa 10% pada Pemeriksaan Mikroskop Malaria. Literature Review, 6–10.

Ridwani, S. (2024). Kualitas Pewarnaan Sediaan Apus Darah Tepi (SADT) Menggunakan Pewarna Giemsa Tanpa Pengenceran. Jurnal STIKes Karsa Husada, 22.

Rinny, A., & Sherly Rosalinda. (2018). Morfologi Eosinofil Pada Apusan Darah Tepi Menggunakan Pewarnaan Giemsa, Wright, Dan Kombinasi Wright-Giemsa. Jurnal Surya Medika, 3(2), 5–12.

Sugiyono. (2017). Metode Penelitian Pendidikan:(Pendekatan Kuantitatif, Kualitatif dan R & D). In Alfabeta (pp. 21–45).

Victoria, Y., Slamet, & Supriyanto. (2019). Analisa Sel Basofil Pada Sediaan Apus Darah Tepi dengan Metode Pewarnaan Giemssa, Wright dan Modifikasi Wright Giemsa. Jurnal Laboratorium Khatulistiwa, 2(1), 8–11.

Vu, Q. H., Van, H. T., Tran, V. T., Huynh, T. D. P., Nguyen, V. C., & Le, D. T. (2021) Development of a Robust Blood Smear Preparation Procedure for External Quality Assessment. Practical Laboratory Medicine, 27(June), e00253.

Wantini, S., & Huda, M. (2021). Pengaruh Konsentrasi dan Waktu Pengecatan Giemsa pada Pemeriksaan Mikroskopik Malaria. Jurnal Analis Kesehatan, 10(1).

Yati, M., Muhlisin, A., Muntaha, A., & Roebiakto, E. (2023). Kualitas Pewarnaan Sediaan Apusan Darah Metode Giemsa Menggunakan Alternatif Pewarna Buah Naga Pengencer Air Mineral. Jurnal Karya Generasi Sehat.




DOI: https://doi.org/10.33559/err.v5i10.3910

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